The most common cause of contaminated samples

It is easily contaminated

The most common cause of contaminated samples is the use of a diluted sample of the human blood. The Glycosylated Hemoglobin Assay Kit is designed to measure the fractional amount of glycosylated hemoglobin in human blood. The amount of glycosylated hemoglobin detected in a sample is determined by comparing the absorbance of the fraction at a given wavelength to the absorbance of the same fraction in a diluted sample of the blood.

A good quality Glycosylated Hemoglobun Assay Kit should be sterile and able to detect contaminates. It is recommended to collect the sample at least 30 minutes after waking up. It is best to collect the blood sample on the 19th, 20th or 21st day of the menstrual cycle, or five days prior to expected menstruation. Male subjects can collect the sample on any day of the month.

It contains sodium azide

The Glycosylated Hemoglobine Assay Kit includes a standard that contains a recombinant or naturally occurring protein. It is expressed in a mammal cell or E.coli. The standard may include a preservative, proclin 300, or both. A wash solution that contains TBS and a stop solution containing sulfuric acid are included in the assay kit. The standard diluent contains 0.02% sodium azide. It may also contain BSA or other antimicrobial agent.

The Assay Kit contains three samples that were tested twenty times in twenty assays. The sample must contain sodium azide and an acid solution such as Tris-HCl. The Stop Solution is included in the kit and should be used within 5 days. Samples after five days should be stored at -20 or -80degC. Grossly hemolyzed samples are not suitable for the assay.