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L-Fucose is present as the main component of fucoidan

Alpha-L-Fucosidase (AFU) is an enzyme coded by the FUCA1 gene that catalyzes the breakdown of L-Fucose. A genetic deficiency in this enzyme leads to neurovisceral storage disease, fucosidosis. Low serum activity of this enzyme has been linked to ovarian caricinoma and elevated activity is associated with diabetes, hyperthyroidism, cirrhosis and hepatitis.
Product Description
Alpha-L-Fucosidase (FUCA1) is an essential enzyme found in the human brain, intestines and kidney that breaks down the polysaccharide fucoidan. A deficiency in this enzyme leads to a neurovisceral storage disease, fucosidosis. High serum activity of a-L-fucosidase is associated with breast, lung, stomach and ovary cancer. It can also be present in patients with diabetes, hyperthyroidism and cirrhosis of the liver. This product allows rapid and sensitive detection of a-L-fucosidase in plasma, serum, tissue or cell lysate. The colorimetric assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. The increase in absorbance at A405 nm is directly proportional to a-L-fucosidase activity.
Detection Range
L-Fucose is present as the main component of fucoidan (a marine polysaccharide), foods, pharmaceuticals and other materials. This colorimetric assay kit is a quick and reliable method for the measurement of?-L-Fucosidase activity, which is the enzyme responsible for the breakdown of L-Fucose. It utilizes a non-radioactive, colorimetric reaction that uses the cleavage of 4-nitrophenol from a synthetic substrate to produce a chromogenic molecule that is detected kinetically at 405 nm. The simple add-mix-read procedure allows the user to quantify fucosidase activity within 20 minutes, making it a great choice for highthroughput liquid handling systems.
Storage
The kit is shipped with blue ice to ensure safe storage of test reagents at -20 degC upon receiving. All components should not be exposed to heat or strong light during storage and usage. This assay kit contains 100 tests for?-L-Fucosidase activity and is based on a colorimetric (405nm) method. This non-radioactive, kinetic method is based on the cleavage of a synthetic substrate containing 4-nitrophenol to release an intensely colored chlorinated p-nitrophenol derivative. It is a rapid and accurate assay with no need for a stop solution, allowing high throughput testing in serum, plasma, tissue or cell lysate. Linear detection range of 1 to 100 U/L.

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