A Brief Overview of the Alkaline Phosphatase Assay Kit (Alp)

An important part of any good laboratory include the various types of sample prep tools and products. The most common types of sample preparation tools are broth mixers, silica electrophoresis/X-ray diffuse, glassware acid-based culture dishes and microplates, wells, and culture drums. All of these products make possible the analysis and evaluation of samples of almost all types. However, these types of products are designed to the specific use that makes it difficult to generalize the usefulness of the various products for various purposes. This is where a reliable alkaline phosphatase in human serum in vitro kit comes in handy.

A reliable alkaline phosphatase in human serum in vitro kit can be used to determine the acid ash retention, colorimetry and liberality of a sample. As soon as samples are mixed with culture medium they undergo several different processes. First, the culture medium is mixed with sample buffer. This combination enables the samples to experience multiple variables of which the buffer is a crucial part. This allows scientists to evaluate the concentrations of different elements on the samples at various times.

An important part of the buffer is the pH indicator. This detects the acidity of the sample to determine the concentration of substrate. Then the next step is the rinsing of the samples to remove any surface residues such as protein or DNA. Next the samples are incubated with varying amounts of alphahydroxyacetone and then are placed in an alkaline phosphate-dissolved culture medium which contains a variety of different biological probes to which alkaline phosphatase has been added.

The kit includes a rinsing brush and micropropulsion pump. The kit uses microcrystalline yeast as a growth medium. Samples can be grown in a Petri dish filled with water or on magnetic plates where the sample is rinsed with distilled water. Frozen human serum or frozen animal serum can also be used to replace the culture media in an alkaline phosphate-dissolved enzyme kit for measuring alp activity in vitro.

There are several advantages to using this type of kit. First, this method is sensitive to changes in substrate concentration thus it can detect any changes in substrate levels as long as the sample remains in suspension. This allows researchers to make measurements of alp kinetics, substrate oxidation and other biochemical processes. Also, the serum samples and the culture medium used in the enzyme assay can be replaced if necessary.

The laboratory reagents in the kit include standard curve reagents and pH indicators. There is also a customized starter kit available. The customized starter kit contains human plasma samples from four different individuals. The blood pressure, pulse rate and temperature are measured as part of the standard curve analysis according to each person.

The pH indicator in the kit is a digital probe that can be used to determine the acidity or alkalinity of human serum or frozen samples. The pH indicator and microcrystalline yeast suspension are combined with the test reagents. After mixing the three components, the samples are incubated for two hours at 37 degrees C. The samples are then run on a linear range of concentrations of the standard curve reagents to determine the sample density and concentration of the alkaline phosphate amine. The microcrystalline yeast is then run on a linear range of concentrations of the substrate amine in the alkaline phosphate amine concentration assays to determine the substrate concentration and density of the alkaline phosphate amine in the samples.

The kit includes an instructional manual with complete instructions on using the kit and the procedure to calculate the reagents needed for use in the tests. The kit can be used for either single or multiple assays. The reagents used are latex based and will not adversely react with any medication that is being taken orally. It is possible to detect 10-250 u alps in samples. For more information on the testing procedures and the sample collection procedures, the manufacturers' websites are available upon request.